Journal: Iranian Journal of Medical Sciences

Article Title: The Adrenal Pheochromocytoma Cell Line PC12 Efficiently Promotes the Regeneration Capability of Adipose Tissue-Derived Mesenchymal Stem Cells in Myogenesis: A Particular Approach to Improving Skeletal Muscle Cell Regeneration

doi: 10.30476/ijms.2023.99642.3175

Figure Lengend Snippet: The morphology of the PC12 cell line was examined when cocultured with differentiated ADSCs. A: PC12 cell line with low density. B: PC12 cell line with high density. C: Transformation of suspended cells into adherent cells. PC12 cell line was adhered to a plastic surface using 0.2 mg/mL collagen. D-F: visualization of adhered PC12 cells. The adhered PC12 cells were visualized using a C7000 cell tracker. The PC12 cells are shown with red colour. The junction between ADSCs and PC12 cells is shown using a black arrow. The connection between two PC12 cells is shown using the yellow arrow. The morphology of the cells was evaluated using the phase contrast microscope with a 200 μm Scale.

Article Snippet: PC12 Cell Line Culture: PC12 cells (C153) were obtained from the cell bank of the Pasteur Institute (Tehran, Iran).

Techniques: Transformation Assay, Microscopy

Journal: Iranian Journal of Medical Sciences

Article Title: The Adrenal Pheochromocytoma Cell Line PC12 Efficiently Promotes the Regeneration Capability of Adipose Tissue-Derived Mesenchymal Stem Cells in Myogenesis: A Particular Approach to Improving Skeletal Muscle Cell Regeneration

doi: 10.30476/ijms.2023.99642.3175

Figure Lengend Snippet: Real-time PCR of Myh1 , Myh2 , and Chrn-α1 genes is illustrated. The coculture process induced a significant increase in Myh1 , Myh2 and Chrn-α1 mRNA expression compared to differentiated ADSCs (A, B, C). P value for Myh1 ; G2 vs. G3: P=0.0134, G2 vs. G4: P=0.0001, G2 vs. G5: P<0.0001, G3 vs. G4: P=0.0080, G3 vs. G5: P<0.0001, G4 vs. G5: P=0.0005, P value for Myh2; G2 vs. G3: P<0.0001, G2 vs. G4: P<0.0001, G2 vs. G5: P<0.0001, G3 vs. G4: P=0.5230, G3 vs. G5: P<0.0001, G4 vs. G5: P=0.0005 and P value for Chrn-α1; G2 vs. G3: P=0.0498, G2 vs. G4 P<0.0001 G2 vs. G5 P<0.0001 G3 vs. G4 P<0.0001 G3 vs. G5 P<0.0001 G4 vs. G5: P=0.8585. Data are presented as mean±SEM of 3 separate experiments. * and # symbols indicate significant differences between the experimental groups G2 group, and coculture groups, respectively. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001; #P<0.05; ##P<0.01; ###P<0.001 and ####P<0.0001. G2; differentiated ADSCs, G3; 30% PC12 cells and 70% of differentiated ADSCs, G4; 50% of the PC12 cells and 50% of differentiated ADSCs, G5; 70% of the PC12 cells and 30% of differentiated ADSCs. Data are presented as mean±SEM of three separate experiments.

Article Snippet: PC12 Cell Line Culture: PC12 cells (C153) were obtained from the cell bank of the Pasteur Institute (Tehran, Iran).

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Iranian Journal of Medical Sciences

Article Title: The Adrenal Pheochromocytoma Cell Line PC12 Efficiently Promotes the Regeneration Capability of Adipose Tissue-Derived Mesenchymal Stem Cells in Myogenesis: A Particular Approach to Improving Skeletal Muscle Cell Regeneration

doi: 10.30476/ijms.2023.99642.3175

Figure Lengend Snippet: Western blot of MYH and nAChR proteins is illustrated. The expression of MYH, and nAChR proteins in differentiated ADSCs cocultured with PC12 cell line. A, B: The expression of MYH and nAChR proteins in group G4 showed significant elevation compared to the control group (G2). However, the expression of MYH and nAChR proteins showed no significant difference with group G2. Protein loading was confirmed by total protein visualization. For MYH; G2 vs. G3: P=0.9630, G2 vs. G4: P=0.0395, G2 vs. G5: P=0.4944, G3 vs. G4: P=0.0206, G3 vs. G5: P=0.7541, G4 vs. G5: P=0.0056 and for nAChR; G2 vs. G3: P=0.0532, G2 vs. G4: P<0.0001, G2 vs. G5: P=0.4603, G3 vs. G4: P=0.0013, G3 vs. G5: P=0.4203, G4 vs. G5: P=0.0003. Data was reported as Mean±SEM for three separate experiments. G2; differentiated ADSCs, G3; 30% of the PC12 cells cocultured with 70% of differentiated ADSCs, G4; 50% of the PC12 cell line cocultured with 50% of differentiated ADSCs, G5; 70% of the PC12 cell line cocultured with 30% of differentiated ADSCs. *P<0.05; ****P<0.0001

Article Snippet: PC12 Cell Line Culture: PC12 cells (C153) were obtained from the cell bank of the Pasteur Institute (Tehran, Iran).

Techniques: Western Blot, Expressing, Control

Journal: Analytical Chemistry

Article Title: Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection

doi: 10.1021/acs.analchem.3c03691

Figure Lengend Snippet: Dose–response curves obtained for the heterogeneous sandwich CRP immunoassays using C153-loaded PSP. The measured emission intensities are normalized to the respective background signal for each assay. These intensities were recorded by measuring (a) the PL intensity of the dye-loaded PSP or (b) the PL signals of the dye molecules released from the particles triggered by extraction with EtOH using 0.1 mg/mL PSP in each experiment. The performance of the differently sized C153-loaded PSP reporters with a PSP concentration of 0.1 mg/mL and different concentrations of 100 nm sized PSP, stained with 10 mM C153 in the CRP immunoassay measured under otherwise identical conditions, is shown in (c) and (d), respectively.

Article Snippet: Coumarin 153 (C153) was obtained from Radiant Dyes Laser GmbH.

Techniques: Extraction, Concentration Assay, Staining

Journal: Analytical Chemistry

Article Title: Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection

doi: 10.1021/acs.analchem.3c03691

Figure Lengend Snippet: Overview of the EC50 Values, Limits of Detection (LOD), Measurement Range, and Relative Dynamic Range (RDR) Obtained for the Different Detection Schemes Using Photoluminescence (PL), Chemiluminescence (CL), or Absorbance (Abs) Measurements

Article Snippet: Coumarin 153 (C153) was obtained from Radiant Dyes Laser GmbH.

Techniques: Enzyme-linked Immunosorbent Assay